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Liquid chromatography apparatus×サーモフィッシャーサイエンティフィック株式会社／Thermo Fisher Scientific K.K. - List of Manufacturers, Suppliers, Companies and Products

Liquid chromatography apparatus Product List

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Transfer of the analysis method for chlorhexidine impurities.

Transfer of the analysis method for chlorhexidine impurities.

We will explain the key points for successfully transferring HPLC analysis methods using the results of rigorous analytical comparisons!

Transferring liquid chromatography (LC) methods between instruments is a challenging task that many analytical laboratories frequently face. The success of this method transfer depends on many factors, making it important to reduce the differences between the systems being transferred and the robustness of the method being transferred. This application note presents a smooth transfer from the Thermo Scientific UltiMate 3000 HPLC system to the Thermo Scientific Vanquish Core HPLC system using an HPLC method from the European Pharmacopoeia.

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  • Analytical Equipment and Devices

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[Case Study] Analysis of Tocopherols α, β, γ, and δ using 2D-LC

[Case Study] Analysis of Tocopherols α, β, γ, and δ using 2D-LC

Vitamin E (alpha, beta, gamma, delta tocopherols) in vegetable oils can be separated and quantified without the influence of interfering components using single-loop heart-cut 2D-LC.

This document introduces a method for the high-precision separation and quantification of four types of tocopherols contained in vegetable oils using the single-loop heart-cut 2D-LC method. Under the separation conditions of a reverse-phase system using a general ODS column, the separation of β-tocopherol and γ-tocopherol is difficult; however, the 2D-LC method allows for easy separation. In the first dimension, tocopherols and interfering components in the sample are separated, and in the second dimension, the four types of tocopherols (α, β, γ, δ) are separated. The repeatability of the standard samples achieved a high reproducibility with RSD < 0.1%. Additionally, measurements conducted on multiple vegetable oils showed that quantification was possible without the influence of interfering peaks. The single-loop heart-cut 2D-LC method is the simplest technique among 2D-LC methods, making it applicable for routine analysis and a useful tool for food analysis involving samples with a high content of matrix components. 【Features】 ■ High-precision separation achieved with the single-loop heart-cut 2D-LC method ■ Separation of β-tocopherol and γ-tocopherol, which is difficult in reverse-phase systems ■ Effective separation of interfering substances in food ■ High reproducibility with repeatability of RSD < 0.1%

  • Analytical Equipment and Devices

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[Case Study] Simultaneous Analysis of Vitamins A, D, and E using 2D-LC

[Case Study] Simultaneous Analysis of Vitamins A, D, and E using 2D-LC

Simplifying the preprocessing with the single loop heart cut 2D-LC method significantly reduces analysis time. It enables the highly sensitive detection of vitamins A, D, E, and their isomers.

This technical document introduces a rapid and quantitative analytical method for the simultaneous detection of seven types of vitamins (vitamin A, vitamin D2, vitamin D3, and four types of vitamin E isomers) in infant formula using a conventional HPLC system with a diode array detector and a fluorescence detector, following offline solid-phase extraction (SPE) and two-dimensional liquid chromatography (2D-LC) analysis. By changing the traditional liquid-liquid extraction method to solid-phase extraction, the time required for sample preparation was reduced from 3 hours to 1 hour. Additionally, by simplifying the sample preparation and optimizing the separation conditions, the influence of the matrix was reduced, allowing for the detection of target substances with higher sensitivity. ■ Simplified sample preparation through two-dimensional liquid chromatography (2D-LC) analysis ■ Simultaneous separation analysis of vitamins A, D, E, and their isomers ■ Developed a method with higher recovery rates and sensitivity than liquid-liquid extraction ■ Simplified the preprocessing process and reduced analysis time by 2.5 times ■ Reduced the influence of the matrix and provided reliable data

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  • Analytical Equipment and Devices

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